In August, the Prague Fertility Centre was the first in the Czech Republic to introduce the method of Laser-Assisted Immotile Sperm Selection (LAISS). This method uses a laser to identify viable immotile sperms for subsequent ICSI, and is recommended in MESA/TESA IVF cycles as well as in other cases of immotile sperms.
Sperm viability is a prerequisite for a successful ICSI treatment, because the injection of a nonviable spermatozoon into an oocyte generally results in fertilization failure. To identify viable sperm in patients presenting with complete sperm immotility is a challenging but very important step. Using LAISS a single laser shot is applied close to the tip of the sperm tail using a non-contact 1.48 µm diode laser system. In a few seconds, two possible reactions can be observed:
- either the tail of the spermatozoa starts curling after the laser shot, or
- the spermatozoa show no reaction at all.
Spermatozoa showing a curling reaction are considered to be viable and can be used for ICSI treatment immediately. In contrast to another methods e.g. hypo-osmotic swelling test, the laser selection can be performed directly in IVF medium with no additional micromanipulation.
LAISS is regarded as a safe procedure since a shot applied to the far end of the flagellum does not affect the sperm head containing the genetic material. Moreover, this novel technique used in a specific group of patients increase the chance to select viable spermatozoa capable of fertilization.
Introduction of LAISS increases the number of treatments on offer at the Prague Fertility Centre as it implements new and progressive methods of IVF therapy.
More information regarding LAISS medium can be found on Prague Fertility Centre’s website.